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KMID : 0368419860290040233
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Journal of Plant Biology
1986 Volume.29 No. 4 p.233 ~ p.242
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Peroxidase Isoxyme in Root Differentiation from Cultured Ginseg Root Explants
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Kim, Myong Won/金明苑
Kim, Chang Mee/Cheong, Hyeon Suk/Kang, Young Hee/金昌美/鄭賢淑/姜榮熹
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Abstract
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In order to pursue some physiological studies on organogenesis in ginseng tissue culture, ginseng root explants were cultured on a modified MS medium containing NAA and kinetin. The activities of peroxidase and some enzymes were investigated and their isoenzyme patterns were also observed. The activity of peroxidase decreased by 20% in one week´s culture and increased thereafter by 80% in culturing for 7 weeks compared with the control group. Glucose-6-phosphate dehydrogenase activity increased after 3 weeks´ culture and 6-phosphogluconate dehydrogenase activity increased by 400% after culturing for 5 weeks and increased during the days preceeding root formation. The activities of glutamate dehydrogenase and acid phosphatase also increased during the culture. After 3 weeks´ culture, new peroxidase isozyme (pH 7.6) appeared and 7 weeks´ culture, another new peroxidase isozyme (pH unidentified) appeared. These patterns were also identified by using FPLC. After 7 weeks´ culture, a new esterase isozyme of pH 8.5 appeared and isozyme patterns of acid phosphatase were quite changed compared with the isozyme patterns of tissue cultured for 5 weeks. In so far as these new isoenzymes appear distinctively after 7 weeks´ culture, root differentiation is supposed to be induced after 7 weeks´ culture.
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